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DNA Labeling at Electron Microscopy

  • Nicolas Thelen
  • Marc ThiryEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1560)

Abstract

Here, we describe a method for locating DNA on ultrathin sections. This technique is compatible with all usual fixation and embedding procedures and can be combined with cytochemical methods. Ultrathin sections are incubated in a medium containing terminal deoxynucleotidyl transferase (TdT) and various non-isotopic nucleotide analogs. The labeled nucleotides bound to the surface of ultrathin sections are then visualized by an indirect immunogold labeling technique. This high-resolution method provides a powerful tool for pinpointing the precise location of DNA within biological material, even where DNA is present in very low amounts.

Key words

Terminal deoxynucleotidyl transferase Immuno-electron microscopy DNA labeling Immunogold techniques Immunocytochemistry 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Giga Neurosciences, Department of Life Sciences, Laboratory of Cell BiologyUniversity of LiègeLiegeBelgium

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