The osmium ammine staining allows the specific detection of DNA in the cell nucleus and represents one of the most used techniques for EM cytochemistry.
The procedure is a Feulgen-type reaction, consisting of an acid hydrolysis to obtain free aldehyde groups on DNA followed by their binding to osmium ammine, a Schiff-type reagent. Osmium ammine is polyamminic electron-dense compound commercially available.
Here, we describe the staining procedure for ultrathin sections and the different procedures for the preparation of the reagent for acrylic and epoxy sections.
DNA staining Osmium ammine Cytochemistry Feulgen reaction Schiff reagent Electron microscopy
This is a preview of subscription content, log in to check access.
Springer Nature is developing a new tool to find and evaluate Protocols. Learn more
The authors would like to thank Ms. Francine Flach for excellent technical skill in preparing the ultrathin sections.
Kasten FH (1960) The chemistry of Schiff’s reagent. Int Rev Cytol 10:1–100Google Scholar
Cogliati R, Gautier A (1973) Demonstration of DNA and polysaccharides using a new Schiff- type reagent. C R Acad Sci 276:3041–3044Google Scholar