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Staining of Lipid Droplets with Monodansylpentane

  • Bo-Hua Chen
  • Huei-Jiun Yang
  • He-Yen Chou
  • Guang-Chao Chen
  • Wei Yuan YangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1560)

Abstract

Monodansylpentane (MDH) is a fluorophore that displays selective labeling of lipid droplets (LDs). The dye preferentially segregates into the neutral lipid cores of LDs and emits blue fluorescence, compatible with the simultaneous use of green and red fluorescent reporters in multi-color live-cell imaging. MDH can be used for visualizing LDs not only in cell cultures, but also in fixed tissues such as the fat body and ovaries from Drosophila. MDH is therefore a versatile marker for LDs in fluorescence microscopy.

Key words

Lipid droplets Fluorescence microscopy Monodansylpentane Live-cell imaging Tissue Drosophila 

Notes

Acknowledgments

This work was supported by the NSC-98-2311-B-001-006-MY3 grant from the National Science Council in Taiwan.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Bo-Hua Chen
    • 1
    • 2
    • 3
  • Huei-Jiun Yang
    • 2
  • He-Yen Chou
    • 2
  • Guang-Chao Chen
    • 1
    • 2
    • 4
  • Wei Yuan Yang
    • 1
    • 2
    • 4
    Email author
  1. 1.Chemical Biology and Molecular Biophysics ProgramTaiwan International Graduate ProgramTaipeiTaiwan
  2. 2.Institute of Biological ChemistryAcademia SinicaNankang, TaipeiTaiwan
  3. 3.Institute of Bioinformatics and Structural BiologyNational Tsing Hua UniversityHsinchuTaiwan
  4. 4.Institute of Biochemical Sciences, College of Life SciencesNational Taiwan UniversityTaipeiTaiwan

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