Staining of Lipid Droplets with Monodansylpentane
Monodansylpentane (MDH) is a fluorophore that displays selective labeling of lipid droplets (LDs). The dye preferentially segregates into the neutral lipid cores of LDs and emits blue fluorescence, compatible with the simultaneous use of green and red fluorescent reporters in multi-color live-cell imaging. MDH can be used for visualizing LDs not only in cell cultures, but also in fixed tissues such as the fat body and ovaries from Drosophila. MDH is therefore a versatile marker for LDs in fluorescence microscopy.
Key wordsLipid droplets Fluorescence microscopy Monodansylpentane Live-cell imaging Tissue Drosophila
This work was supported by the NSC-98-2311-B-001-006-MY3 grant from the National Science Council in Taiwan.