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Nile Red Staining of Neutral Lipids in Yeast

  • Kerry Ann Rostron
  • Clare Louise LawrenceEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1560)

Abstract

Determination of cellular neutral lipid levels in yeast is important for both the biotechnology industry and biomedical research. However, many of the currently available methods are labor intensive and time consuming. Here we describe a rapid and repeatable method for the detection of neutral lipids, which can be utilized in both oleaginous and non-oleaginous yeast species. The method utilizes the fluorescent dye, Nile red, which enables neutral lipid levels to either be visualized via microscopy or quantified using a 96-well plate assay.

Key words

Nile red Neutral lipids Microscopy Microplate Yeast 

Notes

Acknowledgments

This work was supported by The University of Central Lancashire and Brain Tumour North West. The authors would like to acknowledge Dr. Gail Welsby and Dr. Vicky Jones for assistance with microscopy, Mr. Tony Dickson for technical assistance, and Dr. Christopher Smith for support with statistical analysis.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.School of Pharmacy and Biomedical SciencesUniversity of Central LancashirePreston, LancashireUK
  2. 2.School of Biological SciencesUniversity of ReadingReading, BerkshireUK
  3. 3.School of Pharmacy and Biomedical SciencesUniversity of Central LancashirePreston, LancashireUK

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