Nile Red Staining of Neutral Lipids in Yeast
Determination of cellular neutral lipid levels in yeast is important for both the biotechnology industry and biomedical research. However, many of the currently available methods are labor intensive and time consuming. Here we describe a rapid and repeatable method for the detection of neutral lipids, which can be utilized in both oleaginous and non-oleaginous yeast species. The method utilizes the fluorescent dye, Nile red, which enables neutral lipid levels to either be visualized via microscopy or quantified using a 96-well plate assay.
Key wordsNile red Neutral lipids Microscopy Microplate Yeast
This work was supported by The University of Central Lancashire and Brain Tumour North West. The authors would like to acknowledge Dr. Gail Welsby and Dr. Vicky Jones for assistance with microscopy, Mr. Tony Dickson for technical assistance, and Dr. Christopher Smith for support with statistical analysis.
- 8.Shahidi F (2001) Extraction and measurement of total lipids. In current protocols in food analytical chemistry. John Wiley, New York, NYGoogle Scholar