Tissue Fixation and Processing for the Histological Identification of Lipids
Lipids are a heterogeneous group of substances characterized by their solubility in organic solvents and insolubility in water. Lipids can be found as normal components of different tissues and organs, and they can be affected by several pathological conditions. The histochemical identification of lipids plays an important role in histopathological diagnosis and research, but successful staining depends on adequate fixation and processing of the tissue. Here we describe methods to fix and process tissue samples for the histochemical identification of lipids in frozen or paraffin-embedded tissues.
Key wordsLipids fixation Formaldehyde Freezing techniques Sucrose cryoprotection Isopentane Liquid nitrogen Paraffin embedding Linoleic acid Lecithin Lipid histochemistry
The methods described in this chapter were supported by the Spanish “Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica”, from the National Ministry of Economy and Competitiveness (Instituto de Salud Carlos III), Grant No FIS PI14-1343 and cofinanced by “Fondo Europeo de Desarrollo Regional” (FEDER), European Union; The Tissue Engineering Group (CTS-115) of the University of Granada, Spain; and by the “Fundación Benéfica Anticáncer San Francisco Javier y Santa Cándida, Granada, España”.
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