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Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue

  • Sabina BerezowskaEmail author
  • José A. Galván
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1560)

Abstract

Autophagy is a highly conserved cellular mechanism of “self digestion,” ensuring cellular homeostasis, and playing a role in many diseases including cancer. As a stress response mechanism, it may also be involved in cellular response to therapy.

LC3 and Sequestosome 1 (p62/SQSTM1) are among the most widely used markers to monitor autophagy, and can be visualized in formalin-fixed and paraffin-embedded tissue by immunohistochemistry. Here we describe a validated staining protocol using an automated staining system available in many routine pathology laboratories, enabling high-throughput staining under standardized conditions.

Key words

Immunohistochemistry LC3 Sequestosome 1 p62/SQSTM1 Autophagy 

Notes

Acknowledgment

This work was supported by a grant from the Bernese Cancer League to SB.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Institute of PathologyUniversity of BernBernSwitzerland

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