Heat-Induced Antigen Retrieval in Immunohistochemistry: Mechanisms and Applications
Heat-induced antigen retrieval (HIAR) is an essential technique for current immunohistochemistry. We describe the mechanisms of HIAR and its protocols for formalin-fixed, paraffin-embedded specimens and frozen sections, which are the most popular materials for immunohistochemistry using light microscopy. In addition, we describe the antigen retrieval method for highly masked epitopes and double immunostaining using the horseradish peroxidase (HRP)-labeled antibody method and immunofluorescence method in this chapter.
Key wordsHeat-induced antigen retrieval Formalin-fixed, paraffin-embedded tissues Frozen tissues Highly masked epitopes HRP-labeled antibody method Immunofluorescence method Double immunostaining
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