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Characterization of Group 2 Innate Lymphoid Cells in Allergic Airway Inflammation Models in the Mouse

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Inflammation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1559))

Abstract

Allergic asthma is a chronic inflammatory lung disease mediated by type 2 cytokines produced by T helper 2 (Th2) cells as well as the recently discovered group 2 innate lymphoid cells (ILC2). Due to a lack of unique markers, the accurate phenotypic characterization and quantification of ILC2 requires a comprehensive panel of fluorescently labeled antibodies. The markers that are currently used to characterize ILC2 have not been standardized and often vary between research groups, which poses significant challenges when comparing data. Intranasal administration of the pro-inflammatory cytokine IL-33 in mice is associated with strong, Th2 cell-independent ILC2 activation. ILC2 are also activated in mouse models of allergic asthma based on the physiologically relevant house dust mite (HDM) allergen, which parallel eosinophilic airway inflammation observed in asthma patients. Here, we describe the analysis of ILC2 by flow cytometry in these two commonly used allergic airway inflammation models in the mouse.

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Acknowledgments

The authors thank Dr. A. KleinJan (Erasmus MC) for critical reading of the manuscript and Dr. O. Corneth (Erasmus MC) for providing photographs of mouse dissections. This work was partly supported by the Lung Foundation Netherlands (Grant 3.2.12.67).

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Authors and Affiliations

  1. Department of Pulmonary Medicine, Erasmus MC Rotterdam, 2040, 3000, CA, Rotterdam, The Netherlands

    Bobby W. S. Li, Dior M. J. M. Beerens, Maarten D. Brem & Rudi W. Hendriks

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  1. Bobby W. S. Li
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  2. Dior M. J. M. Beerens
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  3. Maarten D. Brem
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  4. Rudi W. Hendriks
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Corresponding author

Correspondence to Rudi W. Hendriks .

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Editors and Affiliations

  1. Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany

    Björn E. Clausen

  2. Section Medical Physiology Department of Neuroscience, University of Groningen University Medical Center Groningen, Groningen, The Netherlands

    Jon D. Laman

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Li, B.W.S., Beerens, D.M.J.M., Brem, M.D., Hendriks, R.W. (2017). Characterization of Group 2 Innate Lymphoid Cells in Allergic Airway Inflammation Models in the Mouse. In: Clausen, B., Laman, J. (eds) Inflammation. Methods in Molecular Biology, vol 1559. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6786-5_12

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  • DOI: https://doi.org/10.1007/978-1-4939-6786-5_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6784-1

  • Online ISBN: 978-1-4939-6786-5

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