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Muscle Stem Cells pp 103–116Cite as

Characterization of Drosophila Muscle Stem Cell-Like Adult Muscle Precursors

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1556))

Abstract

Uncovering how muscle stem cells behave in quiescent and activated states is central to understand the basic rules governing normal muscle development and regeneration in pathological conditions. Specification of mesodermal lineages including muscle stemlike adult muscle precursors (AMPs) has been extensively studied in Drosophila providing an attractive framework for investigating muscle stem cell properties. Restricted number of AMP cells, relative ease in following their behavior, and large number of genetic tools available make fruit fly an attractive model system for studying muscle stem cells. In this chapter, we describe the recently developed tools to visualize and target the body wall and imaginal AMPs.

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Acknowledgments

We wish to thank all members of the Jagla lab for stimulating discussions. We are grateful to former Jagla lab members, N. Figeac, for initial characterization of AMP markers and to R. Aradhya for generating M6-GAL4 and M6-gapGFP lines. This work is supported by the ANR grant ID-CELL-SPE to KJ, the “Equipe” FRM grant to K.J., and the AFM-Téléthon grant to G.L.

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Authors and Affiliations

  1. GReD (Genetics, Reproduction and Development laboratory), INSERM 1103, CNRS 6293, University of Clermont Auvergne, 28 place Henri Dunant, 63000, Clermont-Ferrand, France

    Guillaume Lavergne, Cedric Soler, Monika Zmojdzian & Krzysztof Jagla

Authors
  1. Guillaume Lavergne
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  2. Cedric Soler
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  3. Monika Zmojdzian
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  4. Krzysztof Jagla
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Corresponding author

Correspondence to Krzysztof Jagla .

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Editors and Affiliations

  1. Cell Biology Group, Department of Experimental and Health Sciences, University Pompeu Fabra (UPF), Barcelona, Spain

    Eusebio Perdiguero

  2. Division of Biological Sciences and Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO, USA

    DDW Cornelison

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Lavergne, G., Soler, C., Zmojdzian, M., Jagla, K. (2017). Characterization of Drosophila Muscle Stem Cell-Like Adult Muscle Precursors. In: Perdiguero, E., Cornelison, D. (eds) Muscle Stem Cells. Methods in Molecular Biology, vol 1556. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-6771-1_5

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  • DOI: https://doi.org/10.1007/978-1-4939-6771-1_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-6769-8

  • Online ISBN: 978-1-4939-6771-1

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