Abstract
Bone marrow endothelial progenitor cells (EPCs) have shown a great promise to promote ischemic tissue neovascularization and to attenuate ischemic injury in a variety of animal models, which led to EPC-based clinical trials that yielded modest but promising results. Some of the variables in the use of EPCs relate to their differential isolation and characterization protocols since the EPC literature does not identify a unique marker for these vascular progenitors. In this chapter, we present step-by-step protocols for the isolation of EPCs, their characterization and culture conditions, and their potential use in basic and clinical research.
The original version of this chapter was revised. The erratum to this chapter is available at: DOI 10.1007/978-1-4939-6756-8_26
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Acknowledgments
This work was supported in part by funding from the National Institute of Health grants HL091983, HL095874, HL053354, and HL126186 to (R.K) and American Heart Association Postdoctoral grant to 15POST22720022 (V.N.S.G).
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Garikipati, V.N.S., Kishore, R. (2017). Endothelial Progenitor Cells: Procedure for Cell Isolation and Applications. In: Di Nardo, P., Dhingra, S., Singla, D. (eds) Adult Stem Cells. Methods in Molecular Biology, vol 1553. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6756-8_7
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DOI: https://doi.org/10.1007/978-1-4939-6756-8_7
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