Abstract
Compact bone (cortical or dense bone) is among the organs that contain multipotent mesenchymal stromal cells (MSCs). Unlike bone marrow plugs where MSCs were initially isolated, compact bone has minimal (amount of) hematopoietic cells and thus facilitates the MSCs isolation process. In vitro, MSCs from compact bone show multipotency and differentiation into mesenchymal tissues such as bone, adipose, and cartilage, under certain conditions. MSCs therapy has been promising in preclinical and clinical studies against autoimmune diseases. Not only can MSCs replace the lost tissue through their regenerative properties, but they can also control the autoimmune attacks by immunoregulatory cytokines. This protocol describes the use of compact bone-derived MSCs to preserve salivary function (saliva flow/output) in the NOD (nonobese diabetic) mouse model affected with Sjogren’s-like disease.
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Acknowledgments
The authors are grateful for Dr. Mohammad Bakkar, Dr. Ola Maria, and Mr. Li Chieh Lin for editing the manuscript. The authors are equally grateful for the FACS work previously done by Dr. Ciriaco A. Piccirillo and Dr. Mara Kornete. The authors would like to thank the following funding agency: The University of Jordan, Natural Sciences and Engineering Research Council of Canada (NSERC), and Canadian Institutes of Health Research (CIHR).
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Elghanam, G.A., Liu, Y., Khalili, S., Fang, D., Tran, S.D. (2017). Compact Bone-Derived Multipotent Mesenchymal Stromal Cells (MSCs) for the Treatment of Sjogren’s-like Disease in NOD Mice. In: Di Nardo, P., Dhingra, S., Singla, D. (eds) Adult Stem Cells. Methods in Molecular Biology, vol 1553. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6756-8_3
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DOI: https://doi.org/10.1007/978-1-4939-6756-8_3
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