Abstract
Cardiac regenerative therapy has received attention as a potentially revolutionary approach for treating the damaged heart. The mouse model of myocardial infarction (MI) remains one of the most common tools for the evaluation of such new therapies. Typically, intramyocardial administration of cells or biomaterials in mice is performed by an open-chest surgical procedure, but less invasive delivery methods are becoming available. Echocardiography-based transthoracic myocardial injection is one such minimally invasive approach that can reliably deliver therapeutics to the target site with limited complications and quick recovery for the animal following the procedure. Here, we will describe the method of echocardiography-guided intramyocardial injection in a mouse MI model.
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References
Suuronen EJ, Ruel M (2014) Biomaterials for cardiac regeneration. Springer, New York
Delewi R, Andriessen A, Tijssen JG et al (2013) Impact of intracoronary cell therapy on left ventricular function in the setting of acute myocardial infarction: a meta-analysis of randomised controlled clinical trials. Heart 99:225–232
Jeevanantham V, Butler M, Saad A et al (2012) Adult bone marrow cell therapy improves survival and induces long-term improvement in cardiac parameters: a systematic review and meta-analysis. Circulation 126:551–568
Johnson TD, Christman KL (2013) Injectable hydrogel therapies and their delivery strategies for treating myocardial infarction. Expert Opin Drug Deliv 10:59–72
Mason D, Chen YZ, Krishnan HV et al (2015) Cardiac gene therapy: recent advances and future directions. J Control Release 215:101–111
Patten RD, Hall-Porter MR (2009) Small animal models of heart failure: development of novel therapies, past and present. Circ Heart Fail 2:138–144
Li SH, Lai TY, Sun Z et al (2009) Tracking cardiac engraftment and distribution of implanted bone marrow cells: comparing intra-aortic, intravenous, and intramyocardial delivery. J Thorac Cardiovasc Surg 137(1225–1233):e1221
Blumenthal B, Poppe A, Golsong P et al (2011) Functional regeneration of ischemic myocardium by transplanted cells overexpressing stromal cell-derived factor-1 (SDF-1): intramyocardial injection versus scaffold-based application. Eur J Cardiothorac Surg 40:e135–e141
Bonios M, Terrovitis J, Chang CY et al (2011) Myocardial substrate and route of administration determine acute cardiac retention and lung bio-distribution of cardiosphere-derived cells. J Nucl Cardiol 18:443–450
Hamdi H, Furuta A, Bellamy V et al (2009) Cell delivery: intramyocardial injections or epicardial deposition? A head-to-head comparison. Ann Thorac Surg 87:1196–1203
Ruel M, Shariff MA, Lapierre H et al (2014) Results of the minimally invasive coronary artery bypass grafting angiographic patency study. J Thorac Cardiovasc Surg 147:203–208
Gao E, Lei YH, Shang X et al (2010) A novel and efficient model of coronary artery ligation and myocardial infarction in the mouse. Circ Res 107:1445–1453
Hastings CL, Roche ET, Ruiz-Hernandez E et al (2015) Drug and cell delivery for cardiac regeneration. Adv Drug Deliv Rev 84:85–106
Pompilio G, Steinhoff G, Liebold A et al (2008) Direct minimally invasive intramyocardial injection of bone marrow-derived AC133+ stem cells in patients with refractory ischemia: preliminary results. Thorac Cardiovasc Surg 56:71–76
Ahmadi A, McNeill B, Vulesevic B et al (2014) The role of integrin α2 in cell and matrix therapy that improves perfusion, viability and function of infarcted myocardium. Biomaterials 35:4749–4758
Ahmadi A, Thorn SL, Alarcon EI et al (2015) PET imaging of a collagen matrix reveals its effective injection and targeted retention in a mouse model of myocardial infarction. Biomaterials 49:18–26
Blackburn NJ, Sofrenovic T, Kuraitis D et al (2014) Timing underpins the benefits associated with injectable collagen biomaterial therapy for the treatment of myocardial infarction. Biomaterials 39:182–192
Springer ML, Sievers RE, Viswanathan MN et al (2005) Closed-chest cell injections into mouse myocardium guided by high-resolution echocardiography. Am J Physiol Heart Circ Physiol 289:H1307–H1314
Wang CC, Chen CH, Lin WW et al (2008) Direct intramyocardial injection of mesenchymal stem cell sheet fragments improves cardiac functions after infarction. Cardiovasc Res 77:515–524
Acknowledgments
This work was supported by operating grants from the Canadian Institutes of Health Research (Grant MOP-77536 and Grant FRN 125678 to EJS). KM was supported by the Ottawa Heart Institute Lawrence Soloway Research Fellowship.
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Maeda, K., Seymour, R., Ruel, M., Suuronen, E.J. (2017). Echocardiography-Guided Intramyocardial Injection Method in a Murine Model. In: Di Nardo, P., Dhingra, S., Singla, D. (eds) Adult Stem Cells. Methods in Molecular Biology, vol 1553. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6756-8_17
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DOI: https://doi.org/10.1007/978-1-4939-6756-8_17
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