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Proteome Characterization of a Chromatin Locus Using the Proteomics of Isolated Chromatin Segments Approach

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Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1550))

Abstract

The biological functions of given genomic regions are ruled by the local chromatin composition. The Proteomics of Isolated Chromatin segments approach (PICh) is a powerful and unbiased method to analyze the composition of chosen chromatin segments, provided they are abundant (repeated) or that the organism studied has a small genome. PICh can be used to identify novel and unexpected regulatory factors, or when combined with quantitative mass spectrometric approaches, to characterize the function of a defined factor at the chosen locus, by quantifying composition changes at the locus upon removal/addition of that factor.

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Acknowledgments

We would like to thank Titia de Lange for the kind gift of the TRF1 antibody.

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Authors and Affiliations

  1. INSERM AVENIR, Institute of Human Genetics CNRS UPR1142, 141 rue de la Cardonille, 34000, Montpellier, France

    Sophie L. Kan, Nehmé Saksouk & Jérome Déjardin

Authors
  1. Sophie L. Kan
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  2. Nehmé Saksouk
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  3. Jérome Déjardin
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Corresponding author

Correspondence to Jérome Déjardin .

Editor information

Editors and Affiliations

  1. Keck School of Medicine, University of Southern California, Los Angeles, California, USA

    Lucio Comai

  2. Keck School of Medicine, University of Southern California, Los Angeles, California, USA

    Jonathan E. Katz

  3. Stanford University, Palo Alto, California, USA

    Parag Mallick

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© 2017 Springer Science+Business Media LLC

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Kan, S.L., Saksouk, N., Déjardin, J. (2017). Proteome Characterization of a Chromatin Locus Using the Proteomics of Isolated Chromatin Segments Approach. In: Comai, L., Katz, J., Mallick, P. (eds) Proteomics. Methods in Molecular Biology, vol 1550. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6747-6_3

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  • DOI: https://doi.org/10.1007/978-1-4939-6747-6_3

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6745-2

  • Online ISBN: 978-1-4939-6747-6

  • eBook Packages: Springer Protocols

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