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Methods for MicroRNA Profiling in Cancer

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Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases

Part of the book series: Methods in Pharmacology and Toxicology ((MIPT))

Abstract

MicroRNAs (miRNA) are small non-coding RNAs that negatively regulate post-transcriptional gene expression. Almost all human cancers are characterized by abnormal microRNA expression patterns, which are unique to tumor types. A large body of experimental evidence documents the role of miRNAs in cancer pathogenesis, and specific miRNAs function as oncogenes or tumor suppressors. Due to unique expression profiles and anti/pro-tumorigenic properties of miRNAs, efforts are underway to explore their therapeutic and diagnostic potential. Many miRNA profiling methods have been developed, ranging from Northern blotting and qRT-PCR to the more recent microarray and RNA-Seq platforms. The following chapter details an imaging technique for cellular-specific miRNA expression profiling called in situ hybridization (ISH).

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Author information

Authors and Affiliations

  1. Department of Medical and Molecular Genetics, 2 Simon Cancer Center, Indiana University School of Medicine, IB 545A, 975 West Walnut Street, Indianapolis, IN, 46202-5251, USA

    Sushuma Yarlagadda, Anusha Thota, Ruchi Bansal, Jason Kwon, Murray Korc & Janaiah Kota

Authors
  1. Sushuma Yarlagadda
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  2. Anusha Thota
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  3. Ruchi Bansal
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  4. Jason Kwon
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  5. Murray Korc
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  6. Janaiah Kota
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Corresponding author

Correspondence to Janaiah Kota .

Editor information

Editors and Affiliations

  1. Department of Nutrition Science, Purdue University, West Lafayette, Indiana, USA

    Barbara Stefanska

  2. Department of Molecular and Clinical Pharmacology, Institute of Translational Medicine University of Liverpool, Liverpool, United Kingdom

    David J. MacEwan

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Yarlagadda, S., Thota, A., Bansal, R., Kwon, J., Korc, M., Kota, J. (2017). Methods for MicroRNA Profiling in Cancer. In: Stefanska, B., MacEwan, D. (eds) Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6743-8_7

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  • DOI: https://doi.org/10.1007/978-1-4939-6743-8_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6741-4

  • Online ISBN: 978-1-4939-6743-8

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