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High-Throughput Techniques for DNA Methylation Profiling

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Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases

Part of the book series: Methods in Pharmacology and Toxicology ((MIPT))

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Abstract

In this chapter, commonly used methods to assess the genome-wide DNA methylation status are reviewed and compared. The methods described in this chapter include enrichment-based method, Methylated DNA Immunoprecipitation (MeDIP), paired with microarray technology and next generation sequencing, and sodium bisulfate-based techniques including Infinium HumanMethylation450 BeadChip (Illumina 450 K) and Reduced Representation Bisulfite Sequencing (RRBS).

An overview of each protocol, including description as to why particular steps are required or critical, is outlined. Further, the protocols are compared and advantages and disadvantages of each are discussed.

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Acknowledgments

S.P. is supported by the Mats Sundin Fellowship in Developmental Health. D. C. is supported by fellowship from the Israel Cancer Research Foundation.

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Authors and Affiliations

  1. Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet, Stockholm, Sweden

    Sophie Petropoulos

  2. Department of Pharmacology and Therapeutics, McGill University Medical School, McIntyre Medical Building, 3655 Prom. Sir-William-Osler, Montreal, QC, Canada, H3G 1Y6

    David Cheishvili & Moshe Szyf

Authors
  1. Sophie Petropoulos
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  2. David Cheishvili
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  3. Moshe Szyf
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Corresponding author

Correspondence to Moshe Szyf .

Editor information

Editors and Affiliations

  1. Department of Nutrition Science, Purdue University, West Lafayette, Indiana, USA

    Barbara Stefanska

  2. Department of Molecular and Clinical Pharmacology, Institute of Translational Medicine University of Liverpool, Liverpool, United Kingdom

    David J. MacEwan

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Petropoulos, S., Cheishvili, D., Szyf, M. (2017). High-Throughput Techniques for DNA Methylation Profiling. In: Stefanska, B., MacEwan, D. (eds) Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6743-8_1

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  • DOI: https://doi.org/10.1007/978-1-4939-6743-8_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6741-4

  • Online ISBN: 978-1-4939-6743-8

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