Skip to main content
SpringerLink
Log in

Using Oral and Colon Cancer Cells for Studying the Anticancer Properties of Antimicrobial Peptides

  • Protocol
  • First Online:
Antimicrobial Peptides

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1548))

Abstract

Antimicrobial peptides (AMPs) are of importance in defense mechanism of many organisms and are potential candidate for treatment of infections in animals and humans. AMPs exhibit a wide range of immunomodulatory activities related to innate immunity, wound healing, and inflammation. AMPs also serve as drug delivery vectors, antitumor agents, and mitogenic agents. Here, we describe the investigation of anticancer and cytotoxic activities of antimicrobial peptides by colorimetric MTT assay using smooth muscle, dental pulp stem cell, human colon cancer cell line (SW620), and human oral squamous carcinoma cell line (HSC4).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Cetin Y, Bullerman LB (2005) Cytotoxicity of Fusarium mycotoxins to mammalian cell cultures as determined by the MTT bioassay. Food Chem Toxicol 43:755–764

    Article  CAS  PubMed  Google Scholar 

  2. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63

    Article  CAS  PubMed  Google Scholar 

  3. Riss TL, Moravec RA, Niles AL, Benink HA, Worzella TJ, Minor L (2004) Cell viability assays. In: Sittampalam GS, Coussens NP, Nelson H et al (eds) Assay guidance manual [Internet]. Eli Lilly & Company and the National Center for Advancing Translational Sciences, Bethesda, MD

    Google Scholar 

  4. Lin WJ, Chien YL, Pan CY, Lin TL, Chen JY, Chiu SJ, Hui CF (2009) Epinecidin-1, an antimicrobial peptide from fish (Epinephelus coioides) which has an antitumor effect like lytic peptides in human fibrosarcoma cells. Peptides 30:283–290

    Article  CAS  PubMed  Google Scholar 

  5. Chu HL, Yip BS, Chen KH, Yu HY, Chih YH, Cheng HT, Chou YT, Cheng JW (2015) Novel antimicrobial peptides with high anticancer activity and selectivity. PLoS One 10:e0126390

    Article  PubMed  PubMed Central  Google Scholar 

  6. Wang C, Li HB, Li S, Tian LL, Shang DJ (2012) Antitumor effects and cell selectivity of temporin-1CEa, an antimicrobial peptide from the skin secretions of the Chinese brown frog (Rana chensinensis). Biochimie 94:434–441

    Article  CAS  PubMed  Google Scholar 

  7. Monks A, Scudiero D, Skehan P, Shoemaker R, Paull K, Vistica D, Hose C, Langley J, Cronise P, Vaigro-Wolff A et al (1991) Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines. J Natl Cancer Inst 5(83):757–766

    Article  Google Scholar 

  8. Huang R, Mendis E, Rajapakse N, Kim SK (2006) Strong electronic charge as an important factor for anticancer activity of chitooligosaccharides (COS). Life Sci 11(78):2399–2408

    Article  Google Scholar 

  9. Gronthos S, Arthur A, Mark Bartold P, Shi S (2011) A method to isolate and culture expand human dental pulp stem cells. Methods Mol Biol 698:107–121

    Article  CAS  PubMed  Google Scholar 

  10. Murthy KS, Makhlouf GM (1991) Phosphoinositide metabolism in intestinal smooth muscle: preferential production of Ins (1,4,5) P3 in circular muscle cells. Am J Physiol 261:G945–G951

    CAS  PubMed  Google Scholar 

  11. Murthy KS, Makhlouf GM (1996) Opioid mu, delta, and kappa receptor-induced activation of phospholipase C-beta 3 and inhibition of adenylyl cyclase is mediated by Gi2 and G (o) in smooth muscle. Mol Pharmacol 50:870–877

    CAS  PubMed  Google Scholar 

  12. Sriwai W, Mahavadi S, Al-Shboul O, Grider JR, Murthy KS (2013) Distinctive G protein-dependent signaling by protease-activated receptor 2 (PAR2) in smooth muscle: feedback inhibition of RhoA by cAMP-independent PKA. PLoS One 8:e66743

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgment

This work was supported by TU Research Grant, Thammasat University. Thanks are also extended to the Faculty of Science, Ramkhamhaeng University, and the Faculty of Dentistry, Mahidol University, for their kind support in providing SW620 cell line, oral cancer cell line, and dental pulp stem cells.

Author information

Authors and Affiliations

  1. Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, 99 PaholyotinRoad, Pathumtani, 12121, Thailand

    Teerakul Arpornsuwan & Wimolpak Sriwai

  2. Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, 10400, Thailand

    Hathaitip Sritanaudomchai

  3. Genome Institute, National Center for Genetic Engineering and Biotechnology, Thailand Science Park, Pathumthani, 12121, Thailand

    Sittiruk Roytrakul

Authors
  1. Teerakul Arpornsuwan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wimolpak Sriwai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hathaitip Sritanaudomchai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sittiruk Roytrakul
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Teerakul Arpornsuwan .

Editor information

Editors and Affiliations

  1. Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

    Paul R. Hansen

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer Science+Business Media LLC

About this protocol

Cite this protocol

Arpornsuwan, T., Sriwai, W., Sritanaudomchai, H., Roytrakul, S. (2017). Using Oral and Colon Cancer Cells for Studying the Anticancer Properties of Antimicrobial Peptides. In: Hansen, P. (eds) Antimicrobial Peptides. Methods in Molecular Biology, vol 1548. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6737-7_25

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-6737-7_25

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6735-3

  • Online ISBN: 978-1-4939-6737-7

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation