Using Confocal Microscopy and Computational Modeling to Investigate the Cell-Penetrating Properties of Antimicrobial Peptides

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1548)

Abstract

Antimicrobial peptides (AMPs) may display the ability to penetrate cells, which may be relevant for their antibiotic activity. To investigate the relevance of the penetrating activity for the antibiotic activity of AMPs, here we describe a method based on the combined use of confocal microscopy and computational modeling coupled with cell death kinetics.

Key words

Antimicrobial peptides Cell-penetrating peptides Mathematical modeling Confocal microscopy Multifunctional peptides Cell death kinetics 

Notes

Acknowledgment

We would like to thank Dr. Maria Teresa Lara Ortiz for her technical assistance in the writing of this work. Grants from the Alexander von Humboldt Foundation (FKZ: 3.4-IP-DEU/113263) and PAPIIT (IN205911) to GDR supported this work.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Gabriel Del Rio
    • 1
  • Edda Klipp
    • 2
  • Andreas Herrmann
    • 2
  1. 1.Biochemistry and Structural Biology DepartmentInstituto de Fisiología Celular, Universidad Nacional Autónoma de MéxicoMéxico D.F.Mexico
  2. 2.Theoretische und Molekulare BiophysikHumboldt-Universität zu BerlinBerlinGermany

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