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Microchip Diagnostics pp 143–164Cite as

Droplet-Based Microfluidics Digital PCR for the Detection of KRAS Mutations

Part of the Methods in Molecular Biology book series (MIMB,volume 1547)

Abstract

We demonstrate an accurate and sensitive quantification of mutated KRAS oncogene in genomic DNA, using droplet-based microfluidics and digital PCR.

Key words

  • Droplet microfluidics
  • Digital PCR
  • Biomarkers
  • KRAS
  • Cancer

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  • DOI: 10.1007/978-1-4939-6734-6_12
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Acknowledgments

This work was supported by Région Alsace, the Ministère de l'Enseignement Supérieur et de la Recherche, the Université de Strasbourg, the Université Paris Descartes, the Centre National de la Recherche Scientifique (CNRS), the Institut National de la Santé et de la Recherche Médicale (INSERM), the Institut National du Cancer (INCa, no. 2009-1-RT-03-US-1) and the Association pour la recherche sur le Cancer (ARC, no. SL220100601375).

Author information

Authors and Affiliations

  1. CNRS, Univ. Bordeaux, CRPP, UPR 8641, 115 Avenue Schweitzer, F-33600, Pessac, France

    Deniz Pekin

  2. INSERM UMRS1147, CNRS SNC 5014, Université Paris Descartes, 45 rue des Saints-Pères, 75270, ParisCedex 06, France

    Valerie Taly

Authors
  1. Deniz Pekin
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  2. Valerie Taly
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Corresponding author

Correspondence to Valerie Taly .

Editor information

Editors and Affiliations

  1. INSERM UMRS1147, CNRS SNC 5014, Université Paris Descartes, Equipe labellisée Ligue Nationale contre le cancer 2016, Paris, France

    Valérie Taly

  2. Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, Paris, France

    Jean-Louis Viovy

  3. Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, Paris, France

    Stéphanie Descroix

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Pekin, D., Taly, V. (2017). Droplet-Based Microfluidics Digital PCR for the Detection of KRAS Mutations. In: Taly, V., Viovy, JL., Descroix, S. (eds) Microchip Diagnostics. Methods in Molecular Biology, vol 1547. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6734-6_12

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  • DOI: https://doi.org/10.1007/978-1-4939-6734-6_12

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6732-2

  • Online ISBN: 978-1-4939-6734-6

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