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Microchip Diagnostics pp 133–142Cite as

Multiplex Detection of KRAS Mutations Using Passive Droplet Fusion

Part of the Methods in Molecular Biology book series (MIMB,volume 1547)

Abstract

We describe a droplet microfluidics method to screen for multiple mutations of a same oncogene in a single experiment using passive droplet fusion. Genomic DNA from H1573 cell-line was screened for the presence of the six common mutations of the KRAS oncogene as well as wild-type sequences with a detection efficiency of 98 %. Furthermore, the mutant allelic fraction of the cell-line was also assessed correctly showing that the technique is quantitative

Key words

  • Droplet microfluidics
  • Digital PCR
  • Multiplexing
  • Passive droplet fusion
  • Biomarkers
  • KRAS
  • Cancer

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  • DOI: 10.1007/978-1-4939-6734-6_11
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Acknowledgments

This work was supported by Région Alsace, the Ministère de l’Enseignement Supérieur et de la Recherche, the Université de Strasbourg, the Université Paris Descartes, the Centre National de la Recherche Scientifique (CNRS), the Institut National de la Santé et de la Recherche Médicale (INSERM), the Institut National du Cancer (INCa, no. 2009–1-RT-03-US-1), and the Association pour la recherche sur le Cancer (ARC, no. SL220100601375).

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Authors and Affiliations

  1. CNRS, Univ. Bordeaux, CRPP, UPR 8641, 115 Avenue Schweitzer, F-33600, Pessac, France

    Deniz Pekin

  2. INSERM UMRS1147, CNRS SNC 5014, Université Paris Descartes, Equipe labellisée Ligue Nationale contre le cancer 2016, 45 rue des Saints-Pères, 75270, Paris Cedex 06, France

    Valerie Taly PhD

Authors
  1. Deniz Pekin
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  2. Valerie Taly PhD
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Corresponding author

Correspondence to Valerie Taly PhD .

Editor information

Editors and Affiliations

  1. INSERM UMRS1147, CNRS SNC 5014, Université Paris Descartes, Equipe labellisée Ligue Nationale contre le cancer 2016, Paris, France

    Valérie Taly

  2. Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, Paris, France

    Jean-Louis Viovy

  3. Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, Paris, France

    Stéphanie Descroix

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Pekin, D., Taly, V. (2017). Multiplex Detection of KRAS Mutations Using Passive Droplet Fusion. In: Taly, V., Viovy, JL., Descroix, S. (eds) Microchip Diagnostics. Methods in Molecular Biology, vol 1547. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6734-6_11

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  • DOI: https://doi.org/10.1007/978-1-4939-6734-6_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6732-2

  • Online ISBN: 978-1-4939-6734-6

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