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Multiplex Analysis Using cDNA Transcriptomic Profiling

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Multiplex Biomarker Techniques

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1546))

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Abstract

DNA microarrays contain microscopic DNA spots attached to a solid surface. Each spot contains picomolar levels of a specific DNA probe sequence and hybridization to the corresponding gene products can be detected and quantitated through the use of fluorescently labeled target DNA. In this format, DNA microarrays can be used to measure the expression level of thousands of genes in a single experiment. Here, we present a method to detect the mRNA transcriptional changes in neuronal precursor cells following differentiation using high density cDNA microarrays.

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Author information

Authors and Affiliations

  1. Bioline Reagents Limited, Unit 16, The Edge Business Centre, Humber Road, London, NW2 6EW, UK

    Steve F. C. Hawkins

  2. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil

    Paul C. Guest

Authors
  1. Steve F. C. Hawkins
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  2. Paul C. Guest
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Corresponding author

Correspondence to Steve F. C. Hawkins .

Editor information

Editors and Affiliations

  1. Laboratory of Neuroproteomics, University of Campinas (UNICAMP), Campinas, Brazil

    Paul C. Guest

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Hawkins, S.F.C., Guest, P.C. (2017). Multiplex Analysis Using cDNA Transcriptomic Profiling. In: Guest, P.C. (eds) Multiplex Biomarker Techniques. Methods in Molecular Biology, vol 1546. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-6730-8_9

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  • DOI: https://doi.org/10.1007/978-1-4939-6730-8_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-6729-2

  • Online ISBN: 978-1-4939-6730-8

  • eBook Packages: Springer Protocols

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