Lab-on-a-Chip Multiplex Assays
Lab-on-a-chip multiplex assays allow a rapid identification of multiple parameters in an automated manner. Here we describe a lab-based preparation followed by a rapid and fully automated DNA microarray hybridization and readout in less than 10 min using the Fraunhofer in vitro diagnostics (ivD) platform to enable rapid identification of bacterial species and detection of antibiotic resistance. The use of DNA microarrays allows a fast adaptation of new biomarkers enabling the identification of different genes as well as single-nucleotide-polymorphisms (SNPs) within these genes. In this protocol we describe a DNA microarray developed for identification of Staphylococcus aureus and the mecA resistance gene.
Key wordsBacterial infection Early diagnosis Point-of-care Lab-on-a-chip Antimicrobial drug resistance Antibiotic resistance detection MRSA detection Genotyping SNP detection Automated DNA microarray hybridization
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