Abstract
Proteins and proteomes are dynamic and complex. The accurate identification and measurement of their properties such as abundance, location, and turnover are challenging tasks. Even though high-throughput proteomics has significantly evolved, the technique still lacks fully quantitative and reproducible qualities. A mass spectrometry-based targeted proteomic strategy called selective reaction monitoring (SRM) has emerged in recent years as an important multiplex platform to precisely quantify sets of proteins in multiple samples. This has several advantages in terms of sensitivity, reproducibility, and sample consumption compared to classical methods including those based on antibodies. Here, we present a detailed protocol for quantitation of panels of proteins from cell line extracts using the SRM targeted proteomics approach.
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Acknowledgments
This research was supported by FAPESP (Young Scientist Grant – Proc. No. 2011/0947-1), Center for Cell Based Therapy-CTC-CEPID (Proc. FAPESP 2013/08135-2), and CISBi-NAP. V.M.F. received fellowships from CNPq, Proc. No. 308561/2014-7.
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Faça, V.M. (2017). Selective Reaction Monitoring for Quantitation of Cellular Proteins. In: Guest, P.C. (eds) Multiplex Biomarker Techniques. Methods in Molecular Biology, vol 1546. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-6730-8_18
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DOI: https://doi.org/10.1007/978-1-4939-6730-8_18
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-6729-2
Online ISBN: 978-1-4939-6730-8
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