Abstract
Extracellular vesicles, such as exosomes and microvesicles, have been shown to contain potential microRNA (miRNA) biomarkers that may be utilized in the diagnosis of various diseases from cancer to neurological disorders. The unique nature of the extracellular vesicle bilayer allows miRNA to be protected from degradation making it an ideal source of material for biomarkers discovery from both fresh and archived samples. Here we describe the quantitative analysis of miRNA isolated from exosomes by quantitative PCR and digital PCR.
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Bellingham, S.A., Shambrook, M., Hill, A.F. (2017). Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR. In: Hill, A. (eds) Exosomes and Microvesicles. Methods in Molecular Biology, vol 1545. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6728-5_5
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DOI: https://doi.org/10.1007/978-1-4939-6728-5_5
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6728-5
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