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Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR

  • Shayne A. Bellingham
  • Mitch Shambrook
  • Andrew F. HillEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1545)

Abstract

Extracellular vesicles, such as exosomes and microvesicles, have been shown to contain potential microRNA (miRNA) biomarkers that may be utilized in the diagnosis of various diseases from cancer to neurological disorders. The unique nature of the extracellular vesicle bilayer allows miRNA to be protected from degradation making it an ideal source of material for biomarkers discovery from both fresh and archived samples. Here we describe the quantitative analysis of miRNA isolated from exosomes by quantitative PCR and digital PCR.

Key words

Exosomes miRNA Biomarker Taqman assay RNA isolation Quantitative PCR Digital PCR 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Shayne A. Bellingham
    • 1
    • 2
  • Mitch Shambrook
    • 3
  • Andrew F. Hill
    • 4
    Email author
  1. 1.Department of Biochemistry and Molecular BiologyThe University of MelbourneMelbourneAustralia
  2. 2.Bio21 Molecular Science and Biotechnology InstituteThe University of MelbourneMelbourneAustralia
  3. 3.Department of Biochemistry and Genetics, La Trobe Institute for Molecular ScienceLa Trobe UniversityBundoora, MelbourneAustralia
  4. 4.Department of Biochemistry and Genetics, La Trobe Institute for Molecular ScienceLa Trobe UniversityBundooraAustralia

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