Isolation of Platelet-Derived Extracellular Vesicles
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Platelets participate in several physiological functions, including hemostasis, immunity, and development. Additionally, platelets play key roles in arterial thrombosis and cancer progression. Given this plethora of functions, there is a strong interest of the role of platelet-derived (extracellular) vesicles (PDEVs) as functional mediators and biomarkers. Moreover, the majority of the blood-borne EVs are thought to originate from either platelets or directly from the platelet precursor cells, the megakaryocytes, which reside in the bone marrow. To circumvent confusion, we use the term PDEVs for both platelet-derived and/or megakaryocyte-derived EVs. PDEVs can be isolated from blood or from isolated platelets after activation. In this chapter, we describe all commonly used PDEV isolation methods from blood and prepurified platelets.
Key wordsExtracellular vesicle Platelet Microparticle Exosome Isolation Size-exclusion chromatography Immunobeads Gradient centrifugation
Part of this work was funded by the European Metrology Research Programme (EMRP) under the Joint Research Project HLT02 (www.metves.eu). The EMRP is jointly funded by the EMRP participating countries within the European Association of National Metrology Institutes and the European Union (S.V., Y.Y., and R.N.).
Part of this work was funded by the Magnus Ehrnrooth Foundation, the Medicinska Understödsförening Liv och Hälsa r.f., the Otto Malm Foundation, and the Oscar Öflund Foundation (M.A. and P.S.). Part of the work was funded by the SalWe Research Program GET IT DONE Tekes grant nro 3986/31/2013 (S.V., P.S.).
- 3.van der Pol E, Coumans FA, Grootemaat AE, Gardiner C, Sargent IL, Harrison P et al (2014) Particle size distribution of exosomes and microvesicles determined by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing. J Thromb Haemost 12(7):1182–1192CrossRefPubMedGoogle Scholar
- 9.Ray DM, Spinelli SL, Pollock SJ, Murant TI, O'Brien JJ, Blumberg N et al (2008) Peroxisome proliferator-activated receptor gamma and retinoid X receptor transcription factors are released from activated human platelets and shed in microparticles. Thromb Haemost 99(1):86–95PubMedPubMedCentralGoogle Scholar