Xylem pp 223-231 | Cite as

Structural Analysis of Cell Wall Polysaccharides Using PACE

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1544)

Abstract

The plant cell wall is composed of many complex polysaccharides. The composition and structure of the polysaccharides affect various cell properties including cell shape, cell function and cell adhesion. PACE (Polysaccharide Analysis using Carbohydrate gel Electrophoresis) uses a simple, rapid technique to analyze polysaccharide quantity and structure (Goubet et al., Anal Biochem 300:53–68, 2002).

Key words

Electrophoresis Polysaccharides Glycosyl hydrolases Cell walls Fluorescent labelling 8-aminonaphthalene-1,3,6-trisulfonic acid Xylan 

References

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Joint BioEnergy InstituteLawrence Berkeley National LaboratoryEmeryvilleUSA

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