Structural Analysis of Cell Wall Polysaccharides Using PACE
The plant cell wall is composed of many complex polysaccharides. The composition and structure of the polysaccharides affect various cell properties including cell shape, cell function and cell adhesion. PACE (Polysaccharide Analysis using Carbohydrate gel Electrophoresis) uses a simple, rapid technique to analyze polysaccharide quantity and structure (Goubet et al., Anal Biochem 300:53–68, 2002).
Key wordsElectrophoresis Polysaccharides Glycosyl hydrolases Cell walls Fluorescent labelling 8-aminonaphthalene-1,3,6-trisulfonic acid Xylan
With thanks to Dr. Ramana Pidatala for testing this version of the protocol and to my postdoctoral supervisor, Professor Paul Dupree, whose group developed PACE. This work was part of the DOE Joint BioEnergy Institute (http://www.jbei.org) supported by the U. S. Department of Energy, Office of Science, Office of Biological and Environmental Research, through contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the U. S. Department of Energy..
- 2.Mortimer JC, Miles GP, Brown DM, Zhang Z, Segura MP, Weimar T, Yu X, Seffen KA, Stephens E, Turner SR, Dupree P (2010) Absence of branches from xylan in Arabidopsis gux mutants reveals potential for simplification of lignocellulosic biomass. Proc Natl Acad Sci U S A 107(40):17409–17414. doi: 10.1073/pnas.1005456107 CrossRefPubMedPubMedCentralGoogle Scholar