Abstract
The plant vascular system provides transport and mechanical support functions that are essential for suitable plant growth and development. In Arabidopsis thaliana (Arabidopsis), the vascular tissues at the shoot inflorescence stems are disposed in organized vascular bundles. The vascular patterning emergence and development within the shoot inflorescence stems is under the control of plant growth regulators (De Rybel et al., Nat Rev Mol Cell Biol 17:30–40, 2016; Caño-Delgado et al., Annu Rev Cell Dev Biol 26:605–637, 2010). By using a combined approach of experimental methods for vascular tissues visualization and quantification together with theoretical methods through mathematical and computational modeling, we have reported that auxin transport and brassinosteroid signaling play complementary roles in the formation of the periodic vascular patterning in the shoot (Ibañes et al., Proc Natl Acad Sci U S A 106:13630–13635, 2009; Fàbregas et al., Plant Signal Behav 5:903–906, 2010; Fàbregas et al., PLoS Genet 11:e1005183, 2015). Here, we report the methodology for the interdisciplinary analysis of the shoot vascular patterning in the plant model Arabidopsis into a handle procedure for visualization, quantification, data analysis, and modeling implementation.
Norma Fàbregas and Pau Formosa-Jordan contributed equally to this work
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Acknowledgments
We acknowledge financial support from the Spanish Ministry of Economy and Competitiveness, through the “Severo Ochoa Programme for Centres of Excellence in R&D” 2016–2019 (SEV‐2015‐0533)”. N.F. is funded by “Fundación RENTA CORPORACIÓN” charity in A.C.-D. Lab. P.F.-J. acknowledges the postdoctoral fellowship provided by the Herchel Smith Foundation. P.F.-J and M.I. acknowledge support from the Spanish Ministry of Economy and Competitiveness through grants FIS2012-37655-C02-02 and FIS2015-66503-C3-3-P and to the Generalitat de Catalunya through Projecte Consolidat 2014 SGR 878. A.I.C.-D. lab is funded by a BIO2013-43873 grant from the Spanish Ministry of Economy and Competitiveness, and the European Research Council by the ERC Consolidator Grant (ERC-2015-CoG—683163).
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Fàbregas, N., Formosa-Jordan, P., Ibañes, M., Caño-Delgado, A.I. (2017). Experimental and Theoretical Methods to Approach the Study of Vascular Patterning in the Plant Shoot. In: de Lucas, M., Etchhells, J. (eds) Xylem. Methods in Molecular Biology, vol 1544. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6722-3_1
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DOI: https://doi.org/10.1007/978-1-4939-6722-3_1
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