Abstract
Noncoding RNAs, being at the center stage of the organismal development and homeostasis, warrant a detailed analysis to utilize their full therapeutic potential. They form complexes with various proteins that enable the noncoding RNAs to acquire specific cellular functions such as the transcriptional outcomes that are controlled in a spatio-temporal manner. In this protocol, we describe a method to isolate such known (and unknown) protein complexes bound to a nuclear noncoding RNA.
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Jayani, R.S., Singh, A., Notani, D. (2017). Isolation of Nuclear RNA-Associated Protein Complexes. In: Napoli, S. (eds) Promoter Associated RNA. Methods in Molecular Biology, vol 1543. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6716-2_9
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DOI: https://doi.org/10.1007/978-1-4939-6716-2_9
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6714-8
Online ISBN: 978-1-4939-6716-2
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