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Targeting Conserved Genes in Penicillium Species

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Mycotoxigenic Fungi

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1542))

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Abstract

Polymerase chain reaction amplification of conserved genes and sequence analysis provides a very powerful tool for the identification of toxigenic as well as non-toxigenic Penicillium species. Sequences are obtained by amplification of the gene fragment, sequencing via capillary electrophoresis of dideoxynucleotide-labeled fragments or NGS. The sequences are compared to a database of validated isolates. Identification of species indicates the potential of the fungus to make particular mycotoxins.

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Acknowledgments

Amy E. McGovern very carefully produced high-quality sequences to support this project. The mention of firm names or trade products does not imply that they are endorsed or recommended by the US Department of Agriculture over the firms or similar products not mentioned.

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Correspondence to Stephen W. Peterson .

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Peterson, S.W. (2017). Targeting Conserved Genes in Penicillium Species. In: Moretti, A., Susca, A. (eds) Mycotoxigenic Fungi. Methods in Molecular Biology, vol 1542. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6707-0_9

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  • DOI: https://doi.org/10.1007/978-1-4939-6707-0_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6705-6

  • Online ISBN: 978-1-4939-6707-0

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