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Targeting Conserved Genes in Fusarium Species

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Mycotoxigenic Fungi

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1542))

Abstract

Fumonisins are important mycotoxins contaminating foods and feeds which are mainly produced by F. verticillioides and F. proliferatum. Additionally, both are pathogens of maize and other cereals. We describe two highly sensitive, rapid, and species-specific PCR protocols which enable detection and discrimination of these closely related species in cereal flour or grain samples. The specific primer pairs of these assays were based on the intergenic spacer region of the multicopy rDNA unit which highly improves the sensitivity of the PCR assay in comparison with single-copy target regions.

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Authors and Affiliations

  1. Facultad de Ciencias Biologicas, Departamento de Genetica, Universidad Complutense de Madrid, Jose Antonio Novais 12, Madrid, 28040, Spain

    Miguel Jurado, Salvador Mirete & M. Teresa González-Jaén

  2. Facultad de Ciencias Biologicas, Departamento de Microbiologia, Universidad Complutense de Madrid, Jose Antonio Novais 12, Madrid, 28040, Spain

    Jéssica Gil-Serna, Belén Patiño & Covadonga Vázquez

Authors
  1. Jéssica Gil-Serna
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  2. Belén Patiño
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  3. Miguel Jurado
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  4. Salvador Mirete
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  5. Covadonga Vázquez
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  6. M. Teresa González-Jaén
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Corresponding author

Correspondence to M. Teresa González-Jaén .

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Editors and Affiliations

  1. Institute of Sciences of Food Production National Research Council, Bari, Italy

    Antonio Moretti

  2. Institute of Sciences of Food Production National Research Council, Bari, Italy

    Antonia Susca

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Gil-Serna, J., Patiño, B., Jurado, M., Mirete, S., Vázquez, C., González-Jaén, M.T. (2017). Targeting Conserved Genes in Fusarium Species. In: Moretti, A., Susca, A. (eds) Mycotoxigenic Fungi. Methods in Molecular Biology, vol 1542. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6707-0_8

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  • DOI: https://doi.org/10.1007/978-1-4939-6707-0_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6705-6

  • Online ISBN: 978-1-4939-6707-0

  • eBook Packages: Springer Protocols

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