Abstract
The determination of aflatoxin production ability and differentiation of aflatoxigenic strains can be assessed by monitoring the expression of one or several key genes using reverse transcription polymerase chain reaction (RT-PCR). We herein describe the methods for RNA induction, extraction, and quality determination, and the RT-PCR conditions used to evaluate the ability of a given Aspergillus strain to produce aflatoxins.
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Rodrigues, P.C.A., Gil-Serna, J., González-Jaén, M.T. (2017). Evaluating Aflatoxin Gene Expression in Aspergillus Section Flavi. In: Moretti, A., Susca, A. (eds) Mycotoxigenic Fungi. Methods in Molecular Biology, vol 1542. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6707-0_15
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DOI: https://doi.org/10.1007/978-1-4939-6707-0_15
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6707-0
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