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Analyzing Structural Plasticity of Dendritic Spines in Organotypic Slice Culture

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1538)

Abstract

Understanding the rules of synapse dynamics in the context of development, learning, and nervous system disorders is an important part of several fields of neuroscience. Despite significant methodological advances, observations of structural dynamics of synapses still present a significant experimental challenge. In this chapter we describe a set of techniques that allow repetitive observations of synaptic structures in vitro in organotypic cultures of rodent hippocampus. We describe culturing of slices, transfection with reporter-carrying plasmids, repetitive imaging of dendritic spines with confocal laser scanning microscopy and analysis of spine morphology dynamics.

Key words

Organotypic culture Transfection Confocal imaging Dendritic spine Hippocampus Plasticity Synaptogenesis 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Basic NeuroscienceUniversity of Geneva Medical SchoolGenevaSwitzerland
  2. 2.Department of Anesthesiology, Pharmacology and Intensive CareUniversity Hospitals of GenevaGenevaSwitzerland
  3. 3.Department of NeuroscienceTufts University School of MedicineBostonUSA

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