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Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps

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Oral Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1537))

Abstract

Following the discovery of neutrophil extracellular traps (NETs) in 2004 by Brinkmann and colleagues, there has been extensive research into the role of NETs in a number of inflammatory diseases, including periodontitis. This chapter describes the current methods for the isolation of peripheral blood neutrophils for subsequent NET experiments, including approaches to quantify and visualize NET production, the ability of NETs to entrap and kill bacteria, and the removal of NETs by nuclease-containing plasma.

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Author information

Authors and Affiliations

  1. Institute of Clinical Sciences, College of Medical and Dental Sciences, The School of Dentistry, University of Birmingham, 5 Mill Pool Way, Edgbaston, Birmingham, B5 7EG, UK

    Phillipa C. White, Ilaria J. Chicca, Martin R. Ling, Helen J. Wright, Paul R. Cooper, Mike R. Milward & Iain L. C. Chapple

Authors
  1. Phillipa C. White
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  2. Ilaria J. Chicca
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  3. Martin R. Ling
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  4. Helen J. Wright
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  5. Paul R. Cooper
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  6. Mike R. Milward
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  7. Iain L. C. Chapple
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Corresponding author

Correspondence to Iain L. C. Chapple .

Editor information

Editors and Affiliations

  1. University of Otago Fac. Dentistry, Dunedin, New Zealand

    Gregory J. Seymour

  2. Department of Oral Sciences, University of Otago Faculty of Dentistry, Dunedin, New Zealand

    Mary P. Cullinan

  3. Sir John Walsh Research Institute, University of Otago Faculty of Dentistry, Dunedin, New Zealand

    Nicholas C.K. Heng

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White, P.C. et al. (2017). Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps. In: Seymour, G., Cullinan, M., Heng, N. (eds) Oral Biology. Methods in Molecular Biology, vol 1537. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6685-1_29

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  • DOI: https://doi.org/10.1007/978-1-4939-6685-1_29

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6683-7

  • Online ISBN: 978-1-4939-6685-1

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