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Oral Biology pp 191–202Cite as

Quantitative Analysis of Periodontal Pathogens Using Real-Time Polymerase Chain Reaction (PCR)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1537))

Abstract

The quantitative polymerase chain reaction (qPCR) is a variant of PCR aimed to detect and quantify a targeted DNA molecule through the addition of probes labeled with fluorescent molecules that emit fluorescence within each amplification cycle, what results in fluorescence values proportional to the amount of accumulated PCR product. This chapter presents the detailed procedures for quantification of different periodontal pathogens (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Campylobacter rectus, and Fusobacterium spp.) using qPCR. It also includes the description of the most frequent problems encountered and how to solve them. In addition, a detailed protocol for multiplex qPCR to detect and quantify P. gingivalis and A. actinomycetemcomitans is included.

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Author information

Authors and Affiliations

  1. Oral Research Laboratory, Faculty of Odontology, University Complutense, Madrid, Spain

    Mª José Marin & Elena Figuero

  2. Etiology and Therapy of Periodontal Diseases (ETEP) Research Group, University Complutense, Madrid, Spain

    Elena Figuero, David Herrera & Mariano Sanz

  3. Department of Periodontology, Faculty of Dentistry, University Complutense of Madrid, Plaza Ramón y Cajal, s/n (Ciudad Universitaria), Madrid, 28040, Spain

    Elena Figuero, David Herrera & Mariano Sanz

Authors
  1. Mª José Marin
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  2. Elena Figuero
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  3. David Herrera
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  4. Mariano Sanz
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Corresponding author

Correspondence to Mariano Sanz .

Editor information

Editors and Affiliations

  1. University of Otago Fac. Dentistry, Dunedin, New Zealand

    Gregory J. Seymour

  2. Department of Oral Sciences, University of Otago Faculty of Dentistry, Dunedin, New Zealand

    Mary P. Cullinan

  3. Sir John Walsh Research Institute, University of Otago Faculty of Dentistry, Dunedin, New Zealand

    Nicholas C.K. Heng

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Marin, M.J., Figuero, E., Herrera, D., Sanz, M. (2017). Quantitative Analysis of Periodontal Pathogens Using Real-Time Polymerase Chain Reaction (PCR). In: Seymour, G., Cullinan, M., Heng, N. (eds) Oral Biology. Methods in Molecular Biology, vol 1537. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6685-1_11

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  • DOI: https://doi.org/10.1007/978-1-4939-6685-1_11

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6683-7

  • Online ISBN: 978-1-4939-6685-1

  • eBook Packages: Springer Protocols

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