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Oat pp 31-42 | Cite as

Wide Hybridization Between Oat and Pearl Millet

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1536)

Abstract

Wide hybridization is a one of the important techniques in plant breeding. Oat (Avena sativa L.) and pearl millet (Pennisetum glaucum L.) belong to different subfamilies of Poaceae. In generally, such distant relative species show uniparental chromosome elimination after successful fertilization. However, all seven pearl millet chromosomes are retained beside the genome of oat during embryogenesis. Hybrid seedlings develop, but show necrosis after light irradiation. Here, a detailed protocol for wide hybridization between oat and pearl millet is described.

Key words

Haploid Uniparental chromosome elimination Spike culture Embryo culture 
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Notes

Acknowledgement

I would like to thank Dr. Andreas Houben, IPK, Germany, for reading and providing valuable comments on this manuscript.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)Stadt SeelandGermany

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