Immobilization Techniques of Bacteria for Live Super-resolution Imaging Using Structured Illumination Microscopy

  • Amy L. Bottomley
  • Lynne Turnbull
  • Cynthia B. Whitchurch
  • Elizabeth J. Harry
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1535)

Abstract

Advancements in optical microscopy technology have allowed huge progression in the ability to understand protein structure and dynamics in live bacterial cells using fluorescence microscopy. Paramount to high-quality microscopy is good sample preparation to avoid bacterial cell movement that can result in motion blur during image acquisition. Here, we describe two techniques of sample preparation that reduce unwanted cell movement and are suitable for application to a number of bacterial species and imaging methods.

Key words

Bacterial slide preparation Agarose pads GelGro slabs Super-resolution microscopy Fast three-dimensional structured illumination microscopy 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Amy L. Bottomley
    • 1
  • Lynne Turnbull
    • 1
  • Cynthia B. Whitchurch
    • 1
  • Elizabeth J. Harry
    • 1
  1. 1.The ithree instituteUniversity of Technology SydneySydneyAustralia

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