Abstract
Quantitative evaluation of nanoparticle delivery to a tumor site can be invaluable for cross-platform comparison, a consideration not currently taken into account by many in the field of cancer nanomedicine (Dawidczyk et al., Front Chem 2:69, 2014). Standardization of measured parameters and experimental design will facilitate nanoparticle design and understanding in the field. Here, we present a broadly applicable in vivo protocol for preclinical trials of nanomedicines, including pharmacokinetic modeling and recommendations for parameters to be reported for nanoparticle evaluation. The proposed protocol is simple and not prohibitively mouse-heavy, using procedures that are not overly complicated or difficult to learn, yet is a powerful way to analyze the effectiveness of new cancer nanomedicines against standard or more developed ones.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Dawidczyk CM, Russell LM, Searson PC (2014) Nanomedicines for cancer therapy: state-of-the-art and limitations to pre-clinical studies that hinder future developments. Front Chem 2:69
Dawidczyk CM, Russell LM, Searson PC (2015) Recommendations for benchmarking preclinical studies of nanomedicines. Cancer Res 75(19):4016–4020
Wong AD, Ye M, Ulmschneider MB, Searson PC (2015) Quantitative analysis of the enhanced permeation and retention (EPR) effect. PLoS One 10(5)
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media New York
About this protocol
Cite this protocol
Russell, L.M., Dawidczyk, C.M., Searson, P.C. (2017). Quantitative Evaluation of the Enhanced Permeability and Retention (EPR) Effect. In: Zeineldin, R. (eds) Cancer Nanotechnology. Methods in Molecular Biology, vol 1530. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6646-2_14
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6646-2_14
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6644-8
Online ISBN: 978-1-4939-6646-2
eBook Packages: Springer Protocols