Evolution-Inspired Computational Design of Symmetric Proteins
Monomeric proteins with a number of identical repeats creating symmetrical structures are potentially very valuable building blocks with a variety of bionanotechnological applications. As such proteins do not occur naturally, the emerging field of computational protein design serves as an excellent tool to create them from nonsymmetrical templates. Existing pseudo-symmetrical proteins are believed to have evolved from oligomeric precursors by duplication and fusion of identical repeats. Here we describe a computational workflow to reverse-engineer this evolutionary process in order to create stable proteins consisting of identical sequence repeats.
Key wordsSymmetrical proteins Repeat proteins Rosetta Evolution Ancestral reconstruction Computational protein design
AV acknowledges RIKEN’s program for Junior Scientists for the FPR fellowship and funding.
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