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Antibiotics pp 49–61Cite as

Production of Antimicrobial Compounds by Fermentation

Part of the Methods in Molecular Biology book series (MIMB,volume 1520)

Abstract

The production of biologically active metabolites, e.g., antimicrobial compounds, is an essential step in the discovery and development process of medicinal drugs based on natural products. To get a hand on the compound of interest it first has to be biosynthesized by the corresponding producer, mostly a microorganism. In this chapter, a general workflow, which can easily be adapted to the lab, is described. Both fermentation on solid and in liquid medium is explained, and examples of hand on procedures are given.

Key words

  • Microorganisms
  • Purification
  • Growth media

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Acknowledgment

This work was supported by the German Federal Ministry of Education and Research (BMBF) through the German Centre for Infection Research (DZIF) initiative.

Author information

Authors and Affiliations

  1. Department of Pharmaceutical Biology, University of Bonn, Nußallee 6, 53115, Bonn, Germany

    Henrik Harms, Gabriele M. König & Till F. Schäberle

Authors
  1. Henrik Harms
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  2. Gabriele M. König
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  3. Till F. Schäberle
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Corresponding author

Correspondence to Till F. Schäberle .

Editor information

Editors and Affiliations

  1. Interfaculty Institute for Microbiology and Infection Medicine, Microbial Bioactive Compounds, University of Tübingen, Tübingen, Germany

    Peter Sass

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Harms, H., König, G.M., Schäberle, T.F. (2017). Production of Antimicrobial Compounds by Fermentation. In: Sass, P. (eds) Antibiotics. Methods in Molecular Biology, vol 1520. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6634-9_3

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  • DOI: https://doi.org/10.1007/978-1-4939-6634-9_3

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6632-5

  • Online ISBN: 978-1-4939-6634-9

  • eBook Packages: Springer Protocols

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