Antibiotics pp 281-289 | Cite as

Sample Preparation for Mass-Spectrometry Based Absolute Protein Quantification in Antibiotic Stress Research

  • Florian Bonn
  • Sandra Maass
  • Dörte Becher
Part of the Methods in Molecular Biology book series (MIMB, volume 1520)


Absolute protein quantification is an essential tool for system biology approaches and elucidation of stoichiometry of multi-protein complexes. In this chapter, a universal protocol for gel free absolute protein quantification in bacterial systems is described, which can be used for sample preparation prior to miscellaneous mass-spectrometry-based quantification workflows like AQUA, Hi3, and emPAI. In addition, a focus has been set to the specific challenges in antibiotic stress research.

Key words

Gel free proteomics Sample preparation Absolute protein quantification In-solution digestion AQUA QconCAT Hi3 emPAI Antibiotic stress response 


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Department for Microbial Proteomics, Institute for MicrobiologyErnst-Moritz-Arndt University of GreifswaldGreifswaldGermany

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