Abstract
Expressed protein ligation (EPL) is a valuable tool to study site-specific functionalities on proteins such as posttranslational modifications. The purification of such ligation products from EPL mixtures can be cumbersome due to a small size difference between the expressed protein portion and the desired ligated protein. Therefore, affinity tags are often required, which remain on the protein after purification. Herein, we present an efficient protocol to install a photocleavable biotin building block on synthetic C-terminal tau[390–441] and describe its use for purification of full-length semi-synthetic tau[1–441].
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References
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Reimann, O., Smet-Nocca, C., Hackenberger, C.P.R. (2017). Tag-Free Semi-Synthesis of the Tau Protein. In: Smet-Nocca, C. (eds) Tau Protein. Methods in Molecular Biology, vol 1523. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6598-4_12
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DOI: https://doi.org/10.1007/978-1-4939-6598-4_12
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