Abstract
The advent of human induced pluripotent stem cell (hiPSC) technology has produced patient-specific hiPSC derived cardiomyocytes (hiPSC-CMs) that can be used as a platform to study cardiac diseases and to explore new therapies.
The ability to genetically manipulate hiPSC-CMs not only is essential for identifying the structural and/or functional role of a protein but can also provide valuable information regarding therapeutic applications. In this chapter, we describe protocols for culture, maintenance, and cardiac differentiation of hiPSCs. Then, we provide a basic procedure to transduce hiPSC-CMs.
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Acknowledgments
This work is supported by NIH P50 HL112324, R01 HL119046, R01 HL117505, R01 HL128099, R01 HL129814, R01HL131404, & T32 HL007824 (R. J. H.), a Transatlantic Leducq Foundation grant, and a National Heart, Lung, and Blood Institute Program of Excellence in Nanotechnology award, contract HHSN268201000045C (R.J.H. and 1K99HL104002 (I.K)). We would like to acknowledge the Gene Therapy Resource Program (GTRP) of the National Heart, Lung, and Blood Institute, National Institutes of Health for providing some of the gene vectors used in these studies.
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Stillitano, F., Karakikes, I., Hajjar, R.J. (2017). Gene Transfer in Cardiomyocytes Derived from ES and iPS Cells. In: Ishikawa, K. (eds) Cardiac Gene Therapy. Methods in Molecular Biology, vol 1521. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6588-5_12
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DOI: https://doi.org/10.1007/978-1-4939-6588-5_12
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