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Gene Transfer in Isolated Adult Cardiomyocytes

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Cardiac Gene Therapy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1521))

Abstract

During the past few decades, gene delivery using recombinant virus has made tremendous progress. With a higher than 80 % transduction efficiency, even in non-dividing cells, viral transduction has become the method of choice for efficient gene transfer into cardiomyocytes. However, in vitro gene delivery is dependent on a robust cell isolation protocol, as prolonged cultivation is needed to initiate gene expression and target specific cellular processes. This chapter describes some of the important steps that need to be considered for successful in vitro gene transfer into adult cardiomyocytes. Included are detailed protocols for isolating cells, maintaining rod shaped cardiomyocytes in culture over several days, and employing adenovirus for gene transduction.

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Author information

Authors and Affiliations

  1. Department of Basic Sciences and Aquatic Medicine, Norwegian University of Life Sciences (NMBU), Oslo, Norway

    Kjetil Hodne

  2. Institute for Experimental Medical Research, Oslo University Hospital, University of Oslo, Ullevål, Kirkeveien 166, 0407, Oslo, Norway

    David B. Lipsett & William E. Louch Ph.D.

  3. KG Jebsen Cardiac Research Center and Center for Heart Failure Research, University of Oslo, Oslo, Norway

    David B. Lipsett & William E. Louch Ph.D.

Authors
  1. Kjetil Hodne
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  2. David B. Lipsett
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  3. William E. Louch Ph.D.
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Corresponding author

Correspondence to William E. Louch Ph.D. .

Editor information

Editors and Affiliations

  1. Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, New York, USA

    Kiyotake Ishikawa

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Hodne, K., Lipsett, D.B., Louch, W.E. (2017). Gene Transfer in Isolated Adult Cardiomyocytes. In: Ishikawa, K. (eds) Cardiac Gene Therapy. Methods in Molecular Biology, vol 1521. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6588-5_11

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  • DOI: https://doi.org/10.1007/978-1-4939-6588-5_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6586-1

  • Online ISBN: 978-1-4939-6588-5

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