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Filamentous Bacteria as Targets to Study Phagocytosis

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Phagocytosis and Phagosomes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1519))

Abstract

Filamentous targets are internalized via phagocytic cups that last for several minutes before closing to form a phagosome. This characteristic offers the possibility to study key events in phagocytosis with greater spatial and temporal resolution than is possible to achieve using spherical particles, for which the transition from a phagocytic cup to an enclosed phagosome occurs within a few seconds after particle attachment. In this chapter, we provide methodologies to prepare filamentous bacteria and describe how they can be used as targets to study different aspects of phagocytosis.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON, Canada, M1C 1A4

    Akriti Prashar, Sana I. S. Khan & Mauricio R. Terebiznik Ph.D.

Authors
  1. Akriti Prashar
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  2. Sana I. S. Khan
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  3. Mauricio R. Terebiznik Ph.D.
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Corresponding author

Correspondence to Mauricio R. Terebiznik Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Chemistry and Biology, Ryerson University, Toronto, Ontario, Canada

    Roberto Botelho

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Prashar, A., Khan, S.I.S., Terebiznik, M.R. (2017). Filamentous Bacteria as Targets to Study Phagocytosis. In: Botelho, R. (eds) Phagocytosis and Phagosomes. Methods in Molecular Biology, vol 1519. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6581-6_21

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  • DOI: https://doi.org/10.1007/978-1-4939-6581-6_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6579-3

  • Online ISBN: 978-1-4939-6581-6

  • eBook Packages: Springer Protocols

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