Campylobacter jejuni pp 117-127 | Cite as
Methods to Study Campylobacter jejuni Adherence to and Invasion of Host Epithelial Cells
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Abstract
Measuring bacterial adherence and invasion of cells in vitro has enabled researchers to dissect the interactions of Campylobacter jejuni with eukaryotic cells. Numerous C. jejuni virulence determinants and host cell factors that contribute to the process of adherence, invasion, and immune modulation have been identified utilizing in vitro adherence and invasion assays. In this chapter, we describe the evaluation of C. jejuni adherence to and invasion of HeLa cells using the gentamicin-protection assay.
Key words
Adherence Attachment Binding Invasion Bacteria-host cell interactionsNotes
Acknowledgements
We thank Christopher R. Gourley for critical review of this manuscript and Joanna Fragoso for technical assistance.
Research in the Konkel Laboratory is supported by funds from the United States Department of Agriculture, National Institute of Food and Agriculture (Award Number 2011-67015-30772).
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