Abstract
Here we describe methods for producing nuclei from Arabidopsis suspension cultures or root tips of Arabidopsis, wheat, or pea. These methods could be adapted for other species and cell types. The resulting nuclei can be further purified for use in biochemical or proteomic studies, or can be used for microscopy. We also describe how the nuclei can be used to obtain a preparation of nucleoli.
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Acknowledgements
This work was supported by grant BB/J004588/1 from BBSRC and the John Innes Foundation.
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Pendle, A.F., Shaw, P.J. (2017). Isolation of Nuclei and Nucleoli. In: Taylor, N., Millar, A. (eds) Isolation of Plant Organelles and Structures. Methods in Molecular Biology, vol 1511. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6533-5_3
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DOI: https://doi.org/10.1007/978-1-4939-6533-5_3
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6531-1
Online ISBN: 978-1-4939-6533-5
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