Abstract
This chapter describes a method of plant cytosolic ribosomes isolation typically used for further proteomic studies. Detailed description procedures including plant material disruption, various centrifugation steps, sucrose cushion centrifugation, and quality control of preparation are provided.
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Acknowledgements
This work was supported by grants from the Swedish Research Council and from the Swedish Research Council for Environment, Agriculture and Spatial Planning.
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Klang Årstrand, H., Turkina, M.V. (2017). Isolation of Cytosolic Ribosomes. In: Taylor, N., Millar, A. (eds) Isolation of Plant Organelles and Structures. Methods in Molecular Biology, vol 1511. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6533-5_19
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DOI: https://doi.org/10.1007/978-1-4939-6533-5_19
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