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Microarray Technologies in Fungal Diagnostics

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Human Fungal Pathogen Identification

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1508))

Abstract

Microarray technologies have been a major research tool in the last decades. In addition they have been introduced into several fields of diagnostics including diagnostics of infectious diseases. Microarrays are highly parallelized assay systems that initially were developed for multiparametric nucleic acid detection. From there on they rapidly developed towards a tool for the detection of all kind of biological compounds (DNA, RNA, proteins, cells, nucleic acids, carbohydrates, etc.) or their modifications (methylation, phosphorylation, etc.). The combination of closed-tube systems and lab on chip devices with microarrays further enabled a higher automation degree with a reduced contamination risk. Microarray-based diagnostic applications currently complement and may in the future replace classical methods in clinical microbiology like blood cultures, resistance determination, microscopic and metabolic analyses as well as biochemical or immunohistochemical assays. In addition, novel diagnostic markers appear, like noncoding RNAs and miRNAs providing additional room for novel nucleic acid based biomarkers. Here I focus an microarray technologies in diagnostics and as research tools, based on nucleic acid-based arrays.

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Acknowledgements

This review was made possible through the EU-Project FUNGITECT (Grant no: 602125).

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Authors and Affiliations

  1. Fraunhofer Institut für Grenzflächen- und Bioverfahrenstechnik, Nobelstr. 12, Stuttgart, 70569, Germany

    Steffen Rupp

  2. Institut für Grenzflächenverfahrenstechnik und Plasmatechnologie, Universität Stuttgart, Nobelstr. 12, Stuttgart, 70569, Germany

    Steffen Rupp

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Correspondence to Steffen Rupp .

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  1. Children’s Cancer Research Institute (CCRI), St.Anna Kinderkrebsforschung, Vienna, Austria

    Thomas Lion

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Rupp, S. (2017). Microarray Technologies in Fungal Diagnostics. In: Lion, T. (eds) Human Fungal Pathogen Identification. Methods in Molecular Biology, vol 1508. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6515-1_22

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  • DOI: https://doi.org/10.1007/978-1-4939-6515-1_22

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