Abstract
During cell division the main goal of the cell is to produce two daughter cells with the same genome as the mother, i.e., maintain its genetic stability. Since this issue is essential to preserve the cell ability to proliferate properly, all eukaryotic cells have developed several pathways, called mitotic checkpoints, that regulate mitotic entry, progression, and exit in response to different cellular signals. Given the evolutive conservation of mechanisms and proteins involved in the cell cycle control from yeast to humans, the budding yeast S. cerevisiae has been very helpful to gain insight in these complex regulations. Here, we describe how the checkpoint can be activated and which cellular phenotypes can be used as markers of checkpoint activation.
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Acknowledgments
Thanks to RF laboratory members for useful information. This work was supported by the Italian Ministry of University and Reasearch (PRIN—Progetti di Ricerca di Interesse Nazionale) and by the University of Milano Bicocca.
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Fraschini, R. (2017). Analysis of the Functionality of the Mitotic Checkpoints. In: Monje-Casas, F., Queralt, E. (eds) The Mitotic Exit Network. Methods in Molecular Biology, vol 1505. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6502-1_16
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DOI: https://doi.org/10.1007/978-1-4939-6502-1_16
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Publisher Name: Humana Press, New York, NY
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