Skip to main content
SpringerLink
Log in
Book cover

Mammary Gland Development pp 277–289Cite as

Techniques for the Reprogramming of Exogenous Stem/Progenitor Cell Populations Towards a Mammary Epithelial Cell Fate

  • Protocol
  • First Online:

  • 2271 Accesses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1501))

Abstract

This chapter considers the techniques necessary and required for the reprogramming of exogenous stem/progenitor cell populations towards a mammary epithelial cell fate. The protocols describe how to isolate cells from alternate mouse organs such as testicles of male mice and mix them with mammary cells to generate chimeric glands comprised of male and female epithelial cells that are fully competent. During the reformation of mammary stem cell niches by dispersed epithelial cells, in the context of the intact epithelium-free mammary stroma, non-mammary cells are sequestered and reprogrammed to perform mammary epithelial cell functions including those ascribed to mammary stem/progenitor cells. This therefore is a powerful technique for the redirection of cells from other organs/cancer cells to a normal mammary phenotype.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Daniel CW, DeOme KB, Young JT, Blair PB (1968) The in vivo life span of normal and preneoplastic mouse mammary glands: a serial transplantation study. Proc Natl Acad Sci U S A 61(1):53–60

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Young LJ, Medina D, DeOme KB, Daniel CW (1971) The influence of host and tissue age on life span and growth rate of serially transplanted mouse mammary gland. Exp Gerontol 6:49–56

    Google Scholar 

  3. Daniel CW, Young LJ (1971) Influence of cell division on an aging process. Life span of mouse mammary epithelium during serial propagation in vivo. Exp Cell Res 65:27–32

    Article  CAS  PubMed  Google Scholar 

  4. DeOme KB, Faulkin LJ, Bern HA, Blair PB (1959) Development of mammary tumors from hyperplastic alveolar nodules transplanted into gland-free mammary fat pads of female C3H mice. Cancer Res 19:515–520

    Google Scholar 

  5. Li L, Xie T (2005) Stem cell niche: structure and function. Annu Rev Cell Dev Biol 21:605–631. doi:10.1146/annurev.cellbio.21.012704.131525

    Article  CAS  PubMed  Google Scholar 

  6. Daniel CW, Deome KB (1965) Growth of mouse mammary glands in vivo after monolayer culture. Science 149:634–636

    Article  CAS  PubMed  Google Scholar 

  7. Smith GH (1996) Experimental mammary epithelial morphogenesis in an in vivo model: evidence for distinct cellular progenitors of the ductal and lobular phenotype. Breast Cancer Res Treat 39:21–31

    Article  CAS  PubMed  Google Scholar 

  8. Smith GH, Gallahan D, Zwiebel JA et al (1991) Long-term in vivo expression of genes introduced by retrovirus-mediated transfer into mammary epithelial cells. J Virol 65:6365–6370

    CAS  PubMed  PubMed Central  Google Scholar 

  9. Kordon EC, Smith GH (1998) An entire functional mammary gland may comprise the progeny from a single cell. Development 125:1921–1930

    CAS  PubMed  Google Scholar 

  10. Wagner K-U, Boulanger CA, Henry MD et al (2002) An adjunct mammary epithelial cell population in parous females: its role in functional adaptation and tissue renewal. Development 129:1377–1386

    CAS  PubMed  Google Scholar 

  11. Bellve AR, Millette CF, Bhatnagar YM, O'Brien DA (1977) Dissociation of the mouse testis and characterization of isolated spermatogenic cells. J Histochem Cytochem 25:480–494

    Article  CAS  PubMed  Google Scholar 

  12. Wagner KU, Wall RJ, St-Onge L et al (1997) Cre-mediated gene deletion in the mammary gland. Nucleic Acids Res 25:4323–4330

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Peters SO, Bauermeister K, Simon JP et al (2002) Quantitative polymerase chain reaction-based assay with fluorogenic Y-chromosome specific probes to measure bone marrow chimerism in mice. J Immunol Methods 260:109–116

    Article  CAS  PubMed  Google Scholar 

  14. Rasmussen SB, Kordon E, Callahan R, Smith GH (2001) Evidence for the transforming activity of a truncated Int6 gene, in vitro. Oncogene 20:5291–5301. doi:10.1038/sj.onc.1204624

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The work reported herein was supported in its entirety by the intermural research fund supplied by the Center for Cancer Research, NCI, Bethesda, MD 20892.

Author information

Authors and Affiliations

  1. Mammary Stem Cell Section, Basic Research Laboratory, National Cancer Institute, National Institutes of Health, Building 37 Rm 1112, 37 Convent Drive, Bethesda, MD, 20892, USA

    Gilbert H. Smith & Corinne A. Boulanger

Authors
  1. Gilbert H. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Corinne A. Boulanger
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Gilbert H. Smith or Corinne A. Boulanger .

Editor information

Editors and Affiliations

  1. School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin, Ireland

    Finian Martin

  2. Institute of Cancer Sciences College of MVLS University of Glasgow, Glasgow, United Kingdom

    Torsten Stein

  3. Division of Oncology-Pathology, Lund University Cancer Center/Medicon Village, Lund, Sweden

    Jillian Howlin

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer Science+Business Media New York

About this protocol

Cite this protocol

Smith, G.H., Boulanger, C.A. (2017). Techniques for the Reprogramming of Exogenous Stem/Progenitor Cell Populations Towards a Mammary Epithelial Cell Fate. In: Martin, F., Stein, T., Howlin, J. (eds) Mammary Gland Development. Methods in Molecular Biology, vol 1501. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6475-8_14

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-6475-8_14

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6473-4

  • Online ISBN: 978-1-4939-6475-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation